NG1301 pCI-TPI-4MS2-SMG5(short)-4H
(Plasmid #65806)

• Purpose: NMD reporter mRNA
• Depositing Lab: Niels Gehring
• Publication: Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi: 10.1016/j.celrep.2014.09.012. Epub 2014 Oct 9.

Backbone

• Vector backbone: pCI-neo
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 4572
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: TPI
• Alt name: triosephosphate isomerase 1
• Species: H. sapiens (human)
• Entrez Gene: TPI1 (a.k.a. HEL-S-49, TIM, TPI, TPID)
• Promoter: CMV
• Tags / Fusion Proteins:
  • 4 MS2 stem loops, SMG5 3'UTR shortened version (C terminal on insert)
  • 4H (4 copies of short beta-globin 3'UTR for probe binding) (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EBV-R

Resource Information

• Supplemental Documents:
  • pCI-TPI-4MS2-SMG5(short)-4H genbank

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry