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Addgene

NG0888 pCI-TPI-PTC48-HBB
(Plasmid #65802)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65802 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-neo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4572
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    TPI
  • Alt name
    triosephosphate isomerase 1
  • Species
    H. sapiens (human)
  • Mutation
    premature stop codon at 48
  • Entrez Gene
    TPI1 (a.k.a. HEL-S-49, TIM, TPI, TPID)
  • Promoter CMV
  • Tag / Fusion Protein
    • HBB (beta-globin 3'UTR for probe binding) (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    NG0888 pCI-TPI-PTC48-HBB was a gift from Niels Gehring (Addgene plasmid # 65802 ; http://n2t.net/addgene:65802 ; RRID:Addgene_65802)

  • For your References section:

    Structural and functional analysis of the three MIF4G domains of nonsense-mediated decay factor UPF2. Clerici M, Deniaud A, Boehm V, Gehring NH, Schaffitzel C, Cusack S. Nucleic Acids Res. 2014 Feb;42(4):2673-86. doi: 10.1093/nar/gkt1197. Epub 2013 Nov 23. 10.1093/nar/gkt1197 PubMed 24271394
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