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Purposeexpresses RFP-tagged RNAseH1 in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 65785 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA5/FRT
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Backbone manufacturerLife Technologies, Modified by Tuschl lab
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Vector typeMammalian Expression
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Selectable markersHygromycin, Blasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRNaseH1
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SpeciesH. sapiens (human), D. rerio (zebrafish)
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Entrez GeneRNASEH1 (a.k.a. H1RNA, PEOB2, RNH1)
- Promoter CMV, 2x Tet
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Tags
/ Fusion Proteins
- RFP (N terminal on backbone)
- V5 (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFRT-TODestRFP_RNAseH1 was a gift from Thomas Tuschl (Addgene plasmid # 65785 ; http://n2t.net/addgene:65785 ; RRID:Addgene_65785)