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Addgene

pFRT-TODestFLAGHA_RNAseH1
(Plasmid #65782)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65782 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5/FRT
  • Backbone manufacturer
    Life Technologies, Modified by Tuschl lab
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RNaseH1
  • Species
    H. sapiens (human), D. rerio (zebrafish)
  • Entrez Gene
    RNASEH1 (a.k.a. H1RNA, PEOB2, RNH1)
  • Promoter CMV, 2x Tet
  • Tags / Fusion Proteins
    • FLAG HA (N terminal on backbone)
    • V5 (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFRT-TODestFLAGHA_RNAseH1 was a gift from Thomas Tuschl (Addgene plasmid # 65782 ; http://n2t.net/addgene:65782 ; RRID:Addgene_65782)
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