pFRT-TODestGFP_HuB
(Plasmid
#65761)
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Purposeexpresses GFP-tagged HuB in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 65761 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA5/FRT
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Backbone manufacturerLife Technologies, Modified by Tuschl lab
- Backbone size w/o insert (bp) 5372
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Vector typeMammalian Expression
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Selectable markersHygromycin, Blasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameELAVL2
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SpeciesH. sapiens (human)
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Entrez GeneELAVL2 (a.k.a. HEL-N1, HELN1, HUB)
- Promoter CMV, 2x Tet
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFRT-TODestGFP_HuB was a gift from Thomas Tuschl (Addgene plasmid # 65761 ; http://n2t.net/addgene:65761 ; RRID:Addgene_65761)