pFRT-DestFLAGHA_HuD
(Plasmid
#65760)
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Purposeconstitutively expresses FLAG-HA-tagged HuD in mammalian cells
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 65760 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA5/FRT
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Backbone manufacturerLife Technologies, Modified by Tuschl lab
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameELAVL4
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SpeciesH. sapiens (human)
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Entrez GeneELAVL4 (a.k.a. HUD, PNEM)
- Promoter CMV, 2x Tet
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Tag
/ Fusion Protein
- FLAG HA (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFRT-DestFLAGHA_HuD was a gift from Thomas Tuschl (Addgene plasmid # 65760 ; http://n2t.net/addgene:65760 ; RRID:Addgene_65760)