pFRT-TODestFLAGHA_HuC
(Plasmid #65756)

• Purpose: expresses FLAGHA-tagged HuC in mammalian cells
• Depositing Lab: Thomas Tuschl
• Publication: Identification of the transcriptome-wide mRNA targets of the neuronal ELAVL proteins (unpublished) (unpublished)

Backbone

• Vector backbone: pcDNA5/FRT
• Backbone manufacturer: Life Technologies, Modified by Tuschl lab
• Backbone size w/o insert (bp): 5372
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin, Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: ELAVL3
• Species: H. sapiens (human)
• Entrez Gene: ELAVL3 (a.k.a. HUC, HUCL, PLE21)
• Promoter: CMV, 2x Tet
• Tags / Fusion Proteins:
  • FLAG HA (N terminal on backbone)
  • V5 (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: BGH Reverse

Resource Information

• Supplemental Documents:
  • pFRT-TODestFLAGHA_HuC.sbd
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry