pENTRY4_HuC
(Plasmid
#65753)
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Purposeentry vector with HuC
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 65753 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepENTR 4
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Backbone manufacturerLife Technologies, Modified by Tuschl lab
- Backbone size w/o insert (bp) 2279
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameELAVL3
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SpeciesH. sapiens (human)
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Entrez GeneELAVL3 (a.k.a. HUC, HUCL, PLE21)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer pENTR-F - CTACAAACTCTTCCTGTTAGTTAG
- 3′ sequencing primer pENTR4-R - attttgagacacgggccaga (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pENTRY4_HuC was a gift from Thomas Tuschl (Addgene plasmid # 65753 ; http://n2t.net/addgene:65753 ; RRID:Addgene_65753)