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Addgene

pENTRY4_HuB
(Plasmid #65752)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65752 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pENTR 4
  • Backbone manufacturer
    Life Technologies, Modified by Tuschl lab
  • Backbone size w/o insert (bp) 2279
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ELAVL2
  • Species
    H. sapiens (human)
  • Entrez Gene
    ELAVL2 (a.k.a. HEL-N1, HELN1, HUB)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer pENTR-F - CTACAAACTCTTCCTGTTAGTTAG
  • 3′ sequencing primer pENTR4-R - attttgagacacgggccaga
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pENTRY4_HuB was a gift from Thomas Tuschl (Addgene plasmid # 65752 ; http://n2t.net/addgene:65752 ; RRID:Addgene_65752)
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