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Addgene

pET23a_mod_HuD_FL
(Plasmid #65751)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65751 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pet23a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3392
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ELAVL4
  • Species
    H. sapiens (human)
  • Entrez Gene
    ELAVL4 (a.k.a. HUD, PNEM)
  • Promoter T7
  • Tags / Fusion Proteins
    • FLAG HA (N terminal on backbone)
    • 6X His (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7_promoter_primer
  • 3′ sequencing primer T7_Terminal_Primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET23a_mod_HuD_FL was a gift from Thomas Tuschl (Addgene plasmid # 65751 ; http://n2t.net/addgene:65751 ; RRID:Addgene_65751)
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