pFRT-TODestGFP_RBPMS2
(Plasmid
#65747)
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Purposeexpresses GFP-tagged RBPMS2 in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 65747 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA5/FRT
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Backbone manufacturerLife Technologies, Modified by Tuschl lab
- Backbone size w/o insert (bp) 5372
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Vector typeMammalian Expression
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Selectable markersHygromycin, Blasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameRBPMS2
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SpeciesH. sapiens (human)
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Insert Size (bp)633
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MutationS13N mutation in RBPMS2
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GenBank IDNP_919248.1
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Entrez GeneRBPMS2
- Promoter CMV, 2x Tet
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Tag
/ Fusion Protein
- GFP (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFRT-TODestGFP_RBPMS2 was a gift from Thomas Tuschl (Addgene plasmid # 65747 ; http://n2t.net/addgene:65747 ; RRID:Addgene_65747) -
For your References section:
Identification of the RNA recognition element of the RBPMS family of RNA-binding proteins and their transcriptome-wide mRNA targets. Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, Larsson E, Ohler U, Tuschl T. RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014 May 23. 10.1261/rna.045005.114 PubMed 24860013