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PurposeLentiviral vector for pHRed expression in mammalian cells using the hUbiC promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 65742 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneFUGW
- Backbone size w/o insert (bp) 9200
- Total vector size (bp) 9900
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert namepHRed
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SpeciesSynthetic
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Insert Size (bp)710
- Promoter hUbiC
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer GGTAGGCTCTCCTGAATC (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FUGW-pHRed was a gift from Mathew Tantama (Addgene plasmid # 65742 ; http://n2t.net/addgene:65742 ; RRID:Addgene_65742) -
For your References section:
Imaging energy status in live cells with a fluorescent biosensor of the intracellular ATP-to-ADP ratio. Tantama M, Martinez-Francois JR, Mongeon R, Yellen G. Nat Commun. 2013;4:2550. doi: 10.1038/ncomms3550. 10.1038/ncomms3550 PubMed 24096541