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pet28a_RBPMS_1_144
(Plasmid #65739)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65739 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pet28a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5365
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RBPMS
  • Species
    H. sapiens (human)
  • Mutation
    deletion construct encoding amino acids 1-144
  • GenBank ID
    NM_001008710
  • Entrez Gene
    RBPMS (a.k.a. HERMES)
  • Promoter T7
  • Tag / Fusion Protein
    • His Tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7_promoter_primer
  • 3′ sequencing primer T7_Terminal_Primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pet28a_RBPMS_1_144 was a gift from Thomas Tuschl (Addgene plasmid # 65739 ; http://n2t.net/addgene:65739 ; RRID:Addgene_65739)

  • For your References section:

    Identification of the RNA recognition element of the RBPMS family of RNA-binding proteins and their transcriptome-wide mRNA targets. Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, Larsson E, Ohler U, Tuschl T. RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014 May 23. 10.1261/rna.045005.114 PubMed 24860013
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