pFRT-TODestMYC_RBPMS_KDR
(Plasmid #65667)

• Purpose: MYC expression vector with RBPMS encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)
• Depositing Lab: Thomas Tuschl
• Publication: Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Backbone

• Vector backbone: pcDNA5/FRT/TO
• Backbone manufacturer: Life Technologies, Modified by Tuschl lab
• Backbone size w/o insert (bp): 5372
• Modifications to backbone: introduced HindIII site to exchange FLAG-HA tag for MYC tag
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin, Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RBPMS
• Species: H. sapiens (human)
• Insert Size (bp): 5372
• Mutation: introduced HindIII site to exchange FLAG-HA tag with MYC tag
• GenBank ID: NM_001008710
• Entrez Gene: RBPMS (a.k.a. HERMES)
• Promoter: CMV, 2x Tet
• Tag / Fusion Protein:
  • MYC (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: BGH Reverse

Resource Information

• Supplemental Documents:
  • pFRT-TODestMYC_RBPMS_KDR.sbd

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry