pFRT-TODestMYC_RBPMS_KDR
(Plasmid
#65667)
-
PurposeMYC expression vector with RBPMS encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 65667 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepcDNA5/FRT/TO
-
Backbone manufacturerLife Technologies, Modified by Tuschl lab
- Backbone size w/o insert (bp) 5372
-
Modifications to backboneintroduced HindIII site to exchange FLAG-HA tag for MYC tag
-
Vector typeMammalian Expression
-
Selectable markersHygromycin, Blasticidin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)Top10
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameRBPMS
-
SpeciesH. sapiens (human)
-
Insert Size (bp)5372
-
Mutationintroduced HindIII site to exchange FLAG-HA tag with MYC tag
-
GenBank IDNM_001008710
-
Entrez GeneRBPMS (a.k.a. HERMES)
- Promoter CMV, 2x Tet
-
Tag
/ Fusion Protein
- MYC (N terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pFRT-TODestMYC_RBPMS_KDR was a gift from Thomas Tuschl (Addgene plasmid # 65667 ; http://n2t.net/addgene:65667 ; RRID:Addgene_65667) -
For your References section:
Structural basis underlying CAC RNA recognition by the RRM domain of dimeric RNA-binding protein RBPMS. Teplova M, Farazi TA, Tuschl T, Patel DJ. Q Rev Biophys. 2015 Sep 8:1-11. 10.1017/S0033583515000207 PubMed 26347403