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pFRT-TODestFLAGHA_RBPMS_R38A_E39A
(Plasmid #65664)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65664 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone manufacturer
    Life Technologies, Modified by Tuschl lab
  • Backbone size w/o insert (bp) 5372
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin, Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RBPMS
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    633
  • Mutation
    changed arginine 38 to alanine, and glutamic acid 39 to alanine
  • GenBank ID
    NM_001008710
  • Entrez Gene
    RBPMS (a.k.a. HERMES)
  • Promoter CMV, 2x Tet
  • Tag / Fusion Protein
    • FLAG HA (N terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFRT-TODestFLAGHA_RBPMS_R38A_E39A was a gift from Thomas Tuschl (Addgene plasmid # 65664 ; http://n2t.net/addgene:65664 ; RRID:Addgene_65664)

  • For your References section:

    Structural basis underlying CAC RNA recognition by the RRM domain of dimeric RNA-binding protein RBPMS. Teplova M, Farazi TA, Tuschl T, Patel DJ. Q Rev Biophys. 2015 Sep 8:1-11. 10.1017/S0033583515000207 PubMed 26347403
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