pFRT-TODestFLAGHA_RBPMS_KDR_HindIII
(Plasmid #65662)

• Purpose: FLAG-HA expression vector with RBPMS carrying mutation to introduce HindIII site and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)
• Depositing Lab: Thomas Tuschl
• Publication: Teplova et al Q Rev Biophys. 2015 Sep 8:1-11.

Backbone

• Vector backbone: pcDNA5/FRT/TO
• Backbone manufacturer: Life Technologies, Modified by Tuschl lab
• Backbone size w/o insert (bp): 5372
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin, Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: RBPMS
• Species: H. sapiens (human)
• Insert Size (bp): 633
• Mutation: introduced HindIII site (final goal to exchange FLAG-HA tag with MYC tag)
• GenBank ID: NM_001008710
• Entrez Gene: RBPMS (a.k.a. HERMES)
• Promoter: CMV, 2x Tet
• Tag / Fusion Protein:
  • FLAG HA (N terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: BGH Reverse

Resource Information

• Supplemental Documents:
  • pFRT-TODestFLAGHA_RBPMS_KDR_HindIII.sbd

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry