LRG (Lenti_sgRNA_EFS_GFP) Citations (51)
Originally described in: Discovery of cancer drug targets by CRISPR-Cas9 screening of protein domains.Shi J, Wang E, Milazzo JP, Wang Z, Kinney JB, Vakoc CR Nat Biotechnol. 2015 May 11. doi: 10.1038/nbt.3235. PubMed Journal
Articles Citing LRG (Lenti_sgRNA_EFS_GFP)
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Rapid generation of drug-resistance alleles at endogenous loci using CRISPR-Cas9 indel mutagenesis.
Ipsaro JJ, Shen C, Arai E, Xu Y, Kinney JB, Joshua-Tor L, Vakoc CR, Shi J.
PLoS One. 2017 Feb 23;12(2):e0172177. doi: 10.1371/journal.pone.0172177. eCollection 2017.
PubMed
Associated Plasmids |
Enhancer Reprogramming Promotes Pancreatic Cancer Metastasis.
Roe JS, Hwang CI, Somerville TDD, Milazzo JP, Lee EJ, Da Silva B, Maiorino L, Tiriac H, Young CM, Miyabayashi K, Filippini D, Creighton B, Burkhart RA, Buscaglia JM, Kim EJ, Grem JL, Lazenby AJ, Grunkemeyer JA, Hollingsworth MA, Grandgenett PM, Egeblad M, Park Y, Tuveson DA, Vakoc CR.
Cell. 2017 Aug 24;170(5):875-888.e20. doi: 10.1016/j.cell.2017.07.007. Epub 2017 Jul 27.
PubMed
Associated Plasmids |
| Atg2A/B deficiency switches cytoprotective autophagy to non-canonical caspase-8 activation and apoptosis. Tang Z, Takahashi Y, Chen C, Liu Y, He H, Tsotakos N, Serfass JM, Gebru MT, Chen H, Young MM, Wang HG. Cell Death Differ. 2017 Dec;24(12):2127-2138. doi: 10.1038/cdd.2017.133. Epub 2017 Aug 11. PubMed |
| Promoter-bound METTL3 maintains myeloid leukaemia by m(6)A-dependent translation control. Barbieri I, Tzelepis K, Pandolfini L, Shi J, Millan-Zambrano G, Robson SC, Aspris D, Migliori V, Bannister AJ, Han N, De Braekeleer E, Ponstingl H, Hendrick A, Vakoc CR, Vassiliou GS, Kouzarides T. Nature. 2017 Dec 7;552(7683):126-131. doi: 10.1038/nature24678. Epub 2017 Nov 27. PubMed |
| Enhancer invasion shapes MYCN-dependent transcriptional amplification in neuroblastoma. Zeid R, Lawlor MA, Poon E, Reyes JM, Fulciniti M, Lopez MA, Scott TG, Nabet B, Erb MA, Winter GE, Jacobson Z, Polaski DR, Karlin KL, Hirsch RA, Munshi NP, Westbrook TF, Chesler L, Lin CY, Bradner JE. Nat Genet. 2018 Apr;50(4):515-523. doi: 10.1038/s41588-018-0044-9. Epub 2018 Jan 29. PubMed |
| A Phase I Study of CPI-613 in Combination with High-Dose Cytarabine and Mitoxantrone for Relapsed or Refractory Acute Myeloid Leukemia. Pardee TS, Anderson RG, Pladna KM, Isom S, Ghiraldeli LP, Miller LD, Chou JW, Jin G, Zhang W, Ellis LR, Berenzon D, Howard DS, Hurd DD, Manuel M, Dralle S, Lyerly S, Powell BL. Clin Cancer Res. 2018 May 1;24(9):2060-2073. doi: 10.1158/1078-0432.CCR-17-2282. Epub 2018 Feb 6. PubMed |
| Exploiting genetic variation to uncover rules of transcription factor binding and chromatin accessibility. Behera V, Evans P, Face CJ, Hamagami N, Sankaranarayanan L, Keller CA, Giardine B, Tan K, Hardison RC, Shi J, Blobel GA. Nat Commun. 2018 Feb 22;9(1):782. doi: 10.1038/s41467-018-03082-6. PubMed |
LKB1, Salt-Inducible Kinases, and MEF2C Are Linked Dependencies in Acute Myeloid Leukemia.
Tarumoto Y, Lu B, Somerville TDD, Huang YH, Milazzo JP, Wu XS, Klingbeil O, El Demerdash O, Shi J, Vakoc CR.
Mol Cell. 2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 10.1016/j.molcel.2018.02.011.
PubMed
Associated Plasmids |
| Gastrointestinal stromal tumor enhancers support a transcription factor network predictive of clinical outcome. Hemming ML, Lawlor MA, Zeid R, Lesluyes T, Fletcher JA, Raut CP, Sicinska ET, Chibon F, Armstrong SA, Demetri GD, Bradner JE. Proc Natl Acad Sci U S A. 2018 Jun 19;115(25):E5746-E5755. doi: 10.1073/pnas.1802079115. Epub 2018 Jun 4. PubMed |
| An autophagy assay reveals the ESCRT-III component CHMP2A as a regulator of phagophore closure. Takahashi Y, He H, Tang Z, Hattori T, Liu Y, Young MM, Serfass JM, Chen L, Gebru M, Chen C, Wills CA, Atkinson JM, Chen H, Abraham T, Wang HG. Nat Commun. 2018 Jul 20;9(1):2855. doi: 10.1038/s41467-018-05254-w. PubMed |
| JARID2 Functions as a Tumor Suppressor in Myeloid Neoplasms by Repressing Self-Renewal in Hematopoietic Progenitor Cells. Celik H, Koh WK, Kramer AC, Ostrander EL, Mallaney C, Fisher DAC, Xiang J, Wilson WC, Martens A, Kothari A, Fishberger G, Tycksen E, Karpova D, Duncavage EJ, Lee Y, Oh ST, Challen GA. Cancer Cell. 2018 Nov 12;34(5):741-756.e8. doi: 10.1016/j.ccell.2018.10.008. PubMed |
| Enhancer Architecture and Essential Core Regulatory Circuitry of Chronic Lymphocytic Leukemia. Ott CJ, Federation AJ, Schwartz LS, Kasar S, Klitgaard JL, Lenci R, Li Q, Lawlor M, Fernandes SM, Souza A, Polaski D, Gadi D, Freedman ML, Brown JR, Bradner JE. Cancer Cell. 2018 Dec 10;34(6):982-995.e7. doi: 10.1016/j.ccell.2018.11.001. Epub 2018 Nov 29. PubMed |
| Homolog-Selective Degradation as a Strategy to Probe the Function of CDK6 in AML. Brand M, Jiang B, Bauer S, Donovan KA, Liang Y, Wang ES, Nowak RP, Yuan JC, Zhang T, Kwiatkowski N, Muller AC, Fischer ES, Gray NS, Winter GE. Cell Chem Biol. 2019 Feb 21;26(2):300-306.e9. doi: 10.1016/j.chembiol.2018.11.006. Epub 2018 Dec 27. PubMed |
Engagement of DNA and H3K27me3 by the CBX8 chromodomain drives chromatin association.
Connelly KE, Weaver TM, Alpsoy A, Gu BX, Musselman CA, Dykhuizen EC.
Nucleic Acids Res. 2018 Dec 29. pii: 5266947. doi: 10.1093/nar/gky1290.
PubMed
Associated Plasmids |
| Enhancer Domains in Gastrointestinal Stromal Tumor Regulate KIT Expression and Are Targetable by BET Bromodomain Inhibition. Hemming ML, Lawlor MA, Andersen JL, Hagan T, Chipashvili O, Scott TG, Raut CP, Sicinska E, Armstrong SA, Demetri GD, Bradner JE. Cancer Res. 2019 Mar 1;79(5):994-1009. doi: 10.1158/0008-5472.CAN-18-1888. Epub 2019 Jan 10. PubMed |
| Targeting an RNA-Binding Protein Network in Acute Myeloid Leukemia. Wang E, Lu SX, Pastore A, Chen X, Imig J, Chun-Wei Lee S, Hockemeyer K, Ghebrechristos YE, Yoshimi A, Inoue D, Ki M, Cho H, Bitner L, Kloetgen A, Lin KT, Uehara T, Owa T, Tibes R, Krainer AR, Abdel-Wahab O, Aifantis I. Cancer Cell. 2019 Mar 18;35(3):369-384.e7. doi: 10.1016/j.ccell.2019.01.010. Epub 2019 Feb 21. PubMed |
| The E3 ubiquitin ligase SPOP controls resolution of systemic inflammation by triggering MYD88 degradation. Guillamot M, Ouazia D, Dolgalev I, Yeung ST, Kourtis N, Dai Y, Corrigan K, Zea-Redondo L, Saraf A, Florens L, Washburn MP, Tikhonova AN, Malumbres M, Gong Y, Tsirigos A, Park C, Barbieri C, Khanna KM, Busino L, Aifantis I. Nat Immunol. 2019 Sep;20(9):1196-1207. doi: 10.1038/s41590-019-0454-6. Epub 2019 Aug 12. PubMed |
| Plasticity of the Cullin-RING Ligase Repertoire Shapes Sensitivity to Ligand-Induced Protein Degradation. Mayor-Ruiz C, Jaeger MG, Bauer S, Brand M, Sin C, Hanzl A, Mueller AC, Menche J, Winter GE. Mol Cell. 2019 Aug 22;75(4):849-858.e8. doi: 10.1016/j.molcel.2019.07.013. PubMed |
| Bioactivation of Napabucasin Triggers Reactive Oxygen Species-Mediated Cancer Cell Death. Froeling FEM, Swamynathan MM, Deschenes A, Chio IIC, Brosnan E, Yao MA, Alagesan P, Lucito M, Li J, Chang AY, Trotman LC, Belleau P, Park Y, Rogoff HA, Watson JD, Tuveson DA. Clin Cancer Res. 2019 Dec 1;25(23):7162-7174. doi: 10.1158/1078-0432.CCR-19-0302. Epub 2019 Sep 16. PubMed |
| Macrophages show higher levels of engulfment after disruption of cis interactions between CD47 and the checkpoint receptor SIRPalpha. Hayes BH, Tsai RK, Dooling LJ, Kadu S, Lee JY, Pantano D, Rodriguez PL, Subramanian S, Shin JW, Discher DE. J Cell Sci. 2020 Mar 6;133(5). pii: jcs.237800. doi: 10.1242/jcs.237800. PubMed |
| The Novel Phospholipid Mimetic KPC34 Is Highly Active Against Acute Myeloid Leukemia with Activated Protein Kinase C. Alexander PM, Kucera GL, Pladna KM, Pardee TS. Transl Oncol. 2020 Jul;13(7):100780. doi: 10.1016/j.tranon.2020.100780. Epub 2020 May 16. PubMed |
| Identification of a potent and selective covalent Pin1 inhibitor. Pinch BJ, Doctor ZM, Nabet B, Browne CM, Seo HS, Mohardt ML, Kozono S, Lian X, Manz TD, Chun Y, Kibe S, Zaidman D, Daitchman D, Yeoh ZC, Vangos NE, Geffken EA, Tan L, Ficarro SB, London N, Marto JA, Buratowski S, Dhe-Paganon S, Zhou XZ, Lu KP, Gray NS. Nat Chem Biol. 2020 Sep;16(9):979-987. doi: 10.1038/s41589-020-0550-9. Epub 2020 Jun 1. PubMed |
| Endogenous retroviruses are a source of enhancers with oncogenic potential in acute myeloid leukaemia. Deniz O, Ahmed M, Todd CD, Rio-Machin A, Dawson MA, Branco MR. Nat Commun. 2020 Jul 14;11(1):3506. doi: 10.1038/s41467-020-17206-4. PubMed |
| ZNF410 Uniquely Activates the NuRD Component CHD4 to Silence Fetal Hemoglobin Expression. Lan X, Ren R, Feng R, Ly LC, Lan Y, Zhang Z, Aboreden N, Qin K, Horton JR, Grevet JD, Mayuranathan T, Abdulmalik O, Keller CA, Giardine B, Hardison RC, Crossley M, Weiss MJ, Cheng X, Shi J, Blobel GA. Mol Cell. 2021 Jan 21;81(2):239-254.e8. doi: 10.1016/j.molcel.2020.11.006. Epub 2020 Dec 9. PubMed |
| Mesenchymal stem cells overexpressing BMP-9 by CRISPR-Cas9 present high in vitro osteogenic potential and enhance in vivo bone formation. Freitas GP, Lopes HB, Souza ATP, Gomes MPO, Quiles GK, Gordon J, Tye C, Stein JL, Stein GS, Lian JB, Beloti MM, Rosa AL. Gene Ther. 2021 Dec;28(12):748-759. doi: 10.1038/s41434-021-00248-8. Epub 2021 Mar 8. PubMed |
| Transcriptional Silencing of ALDH2 Confers a Dependency on Fanconi Anemia Proteins in Acute Myeloid Leukemia. Yang Z, Wu XS, Wei Y, Polyanskaya SA, Iyer SV, Jung M, Lach FP, Adelman ER, Klingbeil O, Milazzo JP, Kramer M, Demerdash OE, Chang K, Goodwin S, Hodges E, McCombie WR, Figueroa ME, Smogorzewska A, Vakoc CR. Cancer Discov. 2021 Sep;11(9):2300-2315. doi: 10.1158/2159-8290.CD-20-1542. Epub 2021 Apr 23. PubMed |
| Host factor Rab11a is critical for efficient assembly of influenza A virus genomic segments. Han J, Ganti K, Sali VK, Twigg C, Zhang Y, Manivasagam S, Liang CY, Vogel OA, Huang I, Emmanuel SN, Plung J, Radoshevich L, Perez JT, Lowen AC, Manicassamy B. PLoS Pathog. 2021 May 10;17(5):e1009517. doi: 10.1371/journal.ppat.1009517. eCollection 2021 May. PubMed |
| Longitudinal high-resolution imaging through a flexible intravital imaging window. Jacquemin G, Benavente-Diaz M, Djaber S, Bore A, Dangles-Marie V, Surdez D, Tajbakhsh S, Fre S, Lloyd-Lewis B. Sci Adv. 2021 Jun 16;7(25). pii: 7/25/eabg7663. doi: 10.1126/sciadv.abg7663. Print 2021 Jun. PubMed |
| Rapid interrogation of cancer cell of origin through CRISPR editing. Feng W, Cao Z, Lim PX, Zhao H, Luo H, Mao N, Lee YS, Rivera AA, Choi D, Wu C, Han T, Romero R, de Stanchina E, Carver BS, Wang Q, Jasin M, Sawyers CL. Proc Natl Acad Sci U S A. 2021 Aug 10;118(32). pii: 2110344118. doi: 10.1073/pnas.2110344118. PubMed |
ZMYND8-regulated IRF8 transcription axis is an acute myeloid leukemia dependency.
Cao Z, Budinich KA, Huang H, Ren D, Lu B, Zhang Z, Chen Q, Zhou Y, Huang YH, Alikarami F, Kingsley MC, Lenard AK, Wakabayashi A, Khandros E, Bailis W, Qi J, Carroll MP, Blobel GA, Faryabi RB, Bernt KM, Berger SL, Shi J.
Mol Cell. 2021 Sep 2;81(17):3604-3622.e10. doi: 10.1016/j.molcel.2021.07.018. Epub 2021 Aug 5.
PubMed
Associated Plasmids |
| KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs. Yan F, Li J, Milosevic J, Petroni R, Liu S, Shi Z, Yuan S, Reynaga JM, Qi Y, Rico J, Yu S, Liu Y, Rokudai S, Palmisiano N, Meyer SE, Sung PJ, Wan L, Lan F, Garcia BA, Stanger BZ, Sykes DB, Blanco MA. Cancer Discov. 2022 Mar 1;12(3):792-811. doi: 10.1158/2159-8290.CD-20-1459. PubMed |
| Valine tRNA levels and availability regulate complex I assembly in leukaemia. Thandapani P, Kloetgen A, Witkowski MT, Glytsou C, Lee AK, Wang E, Wang J, LeBoeuf SE, Avrampou K, Papagiannakopoulos T, Tsirigos A, Aifantis I. Nature. 2022 Jan;601(7893):428-433. doi: 10.1038/s41586-021-04244-1. Epub 2021 Dec 22. PubMed |
| SCP4-STK35/PDIK1L complex is a dual phospho-catalytic signaling dependency in acute myeloid leukemia. Polyanskaya SA, Moreno RY, Lu B, Feng R, Yao Y, Irani S, Klingbeil O, Yang Z, Wei Y, Demerdash OE, Benjamin LA, Weiss MJ, Zhang YJ, Vakoc CR. Cell Rep. 2022 Jan 11;38(2):110233. doi: 10.1016/j.celrep.2021.110233. PubMed |
| ZNF204P is a stemness-associated oncogenic long non-coding RNA in hepatocellular carcinoma. Hwang JH, Lee J, Choi WY, Kim MJ, Lee J, Chu KHB, Kim LK, Kim YJ. BMB Rep. 2022 Jun;55(6):281-286. doi: 10.5483/BMBRep.2022.55.6.001. PubMed |
| Bcl-xL Enforces a Slow-Cycling State Necessary for Survival in the Nutrient-Deprived Microenvironment of Pancreatic Cancer. Sela Y, Li J, Maheswaran S, Norgard R, Yuan S, Hubbi M, Doepner M, Xu JP, Ho ES, Mesaros C, Sheehan C, Croley G, Muir A, Blair IA, Shalem O, Dang CV, Stanger BZ. Cancer Res. 2022 May 16;82(10):1890-1908. doi: 10.1158/0008-5472.CAN-22-0431. PubMed |
| Adenosine Monophosphate Activated Protein Kinase (AMPK) enhances chemotherapy response in Acute Myeloid Leukemia (AML). Ghiraldeli L, Anderson R, Pladna K, Pardee TS. Cancer Lett. 2022 Jun 1;535:215659. doi: 10.1016/j.canlet.2022.215659. Epub 2022 Mar 20. PubMed |
| Phase II trial of cytarabine and mitoxantrone with devimistat in acute myeloid leukemia. Anderson R, Miller LD, Isom S, Chou JW, Pladna KM, Schramm NJ, Ellis LR, Howard DS, Bhave RR, Manuel M, Dralle S, Lyerly S, Powell BL, Pardee TS. Nat Commun. 2022 Mar 30;13(1):1673. doi: 10.1038/s41467-022-29039-4. PubMed |
| CD47-SIRPalpha Checkpoint Disruption in Metastases Requires Tumor-Targeting Antibody for Molecular and Engineered Macrophage Therapies. Andrechak JC, Dooling LJ, Tobin MP, Zhang W, Hayes BH, Lee JY, Jin X, Irianto J, Discher DE. Cancers (Basel). 2022 Apr 11;14(8). pii: cancers14081930. doi: 10.3390/cancers14081930. PubMed |
| MOZ and Menin-MLL Complexes Are Complementary Regulators of Chromatin Association and Transcriptional Output in Gastrointestinal Stromal Tumor. Hemming ML, Benson MR, Loycano MA, Anderson JA, Andersen JL, Taddei ML, Krivtsov AV, Aubrey BJ, Cutler JA, Hatton C, Sicinska E, Armstrong SA. Cancer Discov. 2022 Jul 6;12(7):1804-1823. doi: 10.1158/2159-8290.CD-21-0646. PubMed |
| Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programme. Jacquemin G, Wurmser A, Huyghe M, Sun W, Homayed Z, Merle C, Perkins M, Qasrawi F, Richon S, Dingli F, Arras G, Loew D, Vignjevic D, Pannequin J, Fre S. Elife. 2022 May 11;11. pii: 76541. doi: 10.7554/eLife.76541. PubMed |
| NUDT21 limits CD19 levels through alternative mRNA polyadenylation in B cell acute lymphoblastic leukemia. Witkowski MT, Lee S, Wang E, Lee AK, Talbot A, Ma C, Tsopoulidis N, Brumbaugh J, Zhao Y, Roberts KG, Hogg SJ, Nomikou S, Ghebrechristos YE, Thandapani P, Mullighan CG, Hochedlinger K, Chen W, Abdel-Wahab O, Eyquem J, Aifantis I. Nat Immunol. 2022 Oct;23(10):1424-1432. doi: 10.1038/s41590-022-01314-y. Epub 2022 Sep 22. PubMed |
| Functional E3 ligase hotspots and resistance mechanisms to small-molecule degraders. Hanzl A, Casement R, Imrichova H, Hughes SJ, Barone E, Testa A, Bauer S, Wright J, Brand M, Ciulli A, Winter GE. Nat Chem Biol. 2023 Mar;19(3):323-333. doi: 10.1038/s41589-022-01177-2. Epub 2022 Nov 3. PubMed |
| BRD8 maintains glioblastoma by epigenetic reprogramming of the p53 network. Sun X, Klingbeil O, Lu B, Wu C, Ballon C, Ouyang M, Wu XS, Jin Y, Hwangbo Y, Huang YH, Somerville TDD, Chang K, Park J, Chung T, Lyons SK, Shi J, Vogel H, Schulder M, Vakoc CR, Mills AA. Nature. 2023 Jan;613(7942):195-202. doi: 10.1038/s41586-022-05551-x. Epub 2022 Dec 21. PubMed |
| Cell-type-specific prediction of 3D chromatin organization enables high-throughput in silico genetic screening. Tan J, Shenker-Tauris N, Rodriguez-Hernaez J, Wang E, Sakellaropoulos T, Boccalatte F, Thandapani P, Skok J, Aifantis I, Fenyo D, Xia B, Tsirigos A. Nat Biotechnol. 2023 Aug;41(8):1140-1150. doi: 10.1038/s41587-022-01612-8. Epub 2023 Jan 9. PubMed |
| Pyruvate Dehydrogenase Inhibition Leads to Decreased Glycolysis, Increased Reliance on Gluconeogenesis and Alternative Sources of Acetyl-CoA in Acute Myeloid Leukemia. Anderson R, Pladna KM, Schramm NJ, Wheeler FB, Kridel S, Pardee TS. Cancers (Basel). 2023 Jan 12;15(2):484. doi: 10.3390/cancers15020484. PubMed |
| Regulation of human trophoblast gene expression by endogenous retroviruses. Frost JM, Amante SM, Okae H, Jones EM, Ashley B, Lewis RM, Cleal JK, Caley MP, Arima T, Maffucci T, Branco MR. Nat Struct Mol Biol. 2023 Apr;30(4):527-538. doi: 10.1038/s41594-023-00960-6. Epub 2023 Apr 3. PubMed |
| Chromosomal instability can favor macrophage-mediated immune response and induce a broad, vaccination-like anti-tumor IgG response. Hayes BH, Wang M, Zhu H, Phan SH, Andrechak JC, Chang AH, Dooling LJ, Tobin MP, Marchena T, Discher DE. bioRxiv. 2023 Apr 4:2023.04.02.535275. doi: 10.1101/2023.04.02.535275. Preprint. PubMed |
| Acetyl-CoA biosynthesis drives resistance to histone acetyltransferase inhibition. Bishop TR, Subramanian C, Bilotta EM, Garnar-Wortzel L, Ramos AR, Zhang Y, Asiaban JN, Ott CJ, Rock CO, Erb MA. Nat Chem Biol. 2023 May 1. doi: 10.1038/s41589-023-01320-7. PubMed |
| Cathepsin B Is Not an Intrinsic Factor Related to Asparaginase Resistance of the Acute Lymphoblastic Leukemia REH Cell Line. Costa IM, Effer B, Costa-Silva TA, Chen C, Ciccone MF, Pessoa A, Dos Santos CO, Monteiro G. Int J Mol Sci. 2023 Jul 7;24(13):11215. doi: 10.3390/ijms241311215. PubMed |
| RSV replication modifies the XBP1s binding complex on the IRF1 upstream enhancer to potentiate the mucosal anti-viral response. Qiao D, Xu X, Zhang Y, Yang J, Brasier AR. Front Immunol. 2023 Jul 26;14:1197356. doi: 10.3389/fimmu.2023.1197356. eCollection 2023. PubMed |
| Genome scale CRISPR screens identify actin capping proteins as key modulators of therapeutic responses to radiation and immunotherapy. Verma N, Renauer PA, Dong C, Xin S, Lin Q, Zhang F, Glazer PM, Chen S. bioRxiv [Preprint]. 2024 Jan 15:2024.01.14.575614. doi: 10.1101/2024.01.14.575614. PubMed |
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