pIK198
(Plasmid #65629)

• Purpose: (Empty Backbone) "flipped and extended" sgRNA backbone (Chen et al., 2013) following a C. elegans U6 promoter (Friedland et al., 2013). For Gibson cloning locus-specific sgRNA sequences.
• Depositing Lab: Iskra Katic
• Publication: Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.019273. doi: 10.1534/g3.115.019273.

Backbone

• Vector backbone: pUC57
• Backbone manufacturer: Genscript
• Backbone size (bp): 2710
• Modifications to backbone: Cloned a C. elegans U6 promoter and an sgRNA backbone through Gibson cloning
• Vector type: Worm Expression, CRISPR
• Promoter: U6

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL1 Blue
• Copy number: High Copy

Cloning Information

• Cloning method: Gibson Cloning

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The U6 promoter was received from the Calarco lab, through Addgene (#46169). The sgRNA backbone was synthesized by IDT.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry