Degron-KI PIK3CA donor vector
(Plasmid
#65621)
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PurposeDonor vector to generate Degron-KI DD-PIK3CA
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 65621 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backboneDegron-KI donor backbone vector
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Top10
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePIK3CA Degron-KI left and right HR arms
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Degron-KI PIK3CA donor vector was a gift from Frank Stegmeier (Addgene plasmid # 65621 ; http://n2t.net/addgene:65621 ; RRID:Addgene_65621) -
For your References section:
A chemical genetics approach for the functional assessment of novel cancer genes. Zhou Q, Derti A, Ruddy DA, Rakiec DP, Kao I, Lira M, Gibaja V, Chan H, Yang Y, Min J, Schlabach MR, Stegmeier F. Cancer Res. 2015 Mar 18. pii: canres.2930.2014. 10.1158/0008-5472.CAN-14-2930 PubMed 25788694