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Addgene

Degron-KI p53 donor vector
(Plasmid #65619)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65619 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Degron-KI donor backbone vector
  • Modifications to backbone
    HR arms were cloned into Nhe1 and xho1; Not1 and EcoR1 sites. xho1 site was removed in the final vector.
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    p53 Degron-KI left and right HR arms

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Degron-KI p53 donor vector was a gift from Frank Stegmeier (Addgene plasmid # 65619 ; http://n2t.net/addgene:65619 ; RRID:Addgene_65619)

  • For your References section:

    A chemical genetics approach for the functional assessment of novel cancer genes. Zhou Q, Derti A, Ruddy DA, Rakiec DP, Kao I, Lira M, Gibaja V, Chan H, Yang Y, Min J, Schlabach MR, Stegmeier F. Cancer Res. 2015 Mar 18. pii: canres.2930.2014. 10.1158/0008-5472.CAN-14-2930 PubMed 25788694
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