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Addgene

pHis-zfCSF3b
(Plasmid #65613)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65613 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pETH2a
  • Backbone size w/o insert (bp) 4500
  • Total vector size (bp) 4979
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The depositing laboratory recommends strain BL21(DE3) for protein expression.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Csf3b
  • Alt name
    Gcsf3b
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    471
  • GenBank ID
    NM_001143754
  • Entrez Gene
    csf3b (a.k.a. Gcs, Gcsf, csf3)
  • Promoter T7
  • Tag / Fusion Protein
    • (His)6 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHis-zfCSF3b was a gift from Petr Bartunek (Addgene plasmid # 65613 ; http://n2t.net/addgene:65613 ; RRID:Addgene_65613)

  • For your References section:

    The zebrafish granulocyte colony-stimulating factors (Gcsfs): 2 paralogous cytokines and their roles in hematopoietic development and maintenance. Stachura DL, Svoboda O, Campbell CA, Espin-Palazon R, Lau RP, Zon LI, Bartunek P, Traver D. Blood. 2013 Dec 5;122(24):3918-28. doi: 10.1182/blood-2012-12-475392. Epub 2013 Oct 15. 10.1182/blood-2012-12-475392 PubMed 24128862
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