pHis-zfCSF3a
(Plasmid
#65612)
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Purposeexpress soluble His-tagged zebrafish CSF3a/Gcsfa in E. coli
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 65612 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepETH2a
- Backbone size w/o insert (bp) 4500
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsThe depositing laboratory recommends strain BL21(DE3) for protein expression.
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCsf3a
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Alt nameGcsf3a
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SpeciesD. rerio (zebrafish)
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Insert Size (bp)574
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GenBank IDNM_001145242
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Entrez Genecsf3a (a.k.a. csf3, gcsf)
- Promoter T7
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Tag
/ Fusion Protein
- (His)6 (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (unknown if destroyed)
- 3′ cloning site NcoI (unknown if destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHis-zfCSF3a was a gift from Petr Bartunek (Addgene plasmid # 65612 ; http://n2t.net/addgene:65612 ; RRID:Addgene_65612) -
For your References section:
Clonal analysis of hematopoietic progenitor cells in the zebrafish. Stachura DL, Svoboda O, Lau RP, Balla KM, Zon LI, Bartunek P, Traver D. Blood. 2011 Aug 4;118(5):1274-82. doi: 10.1182/blood-2011-01-331199. Epub 2011 Mar 17. 10.1182/blood-2011-01-331199 PubMed 21415264