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Addgene

pHis-zfCSF3a
(Plasmid #65612)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65612 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pETH2a
  • Backbone size w/o insert (bp) 4500
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The depositing laboratory recommends strain BL21(DE3) for protein expression.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Csf3a
  • Alt name
    Gcsf3a
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    574
  • GenBank ID
    NM_001145242
  • Entrez Gene
    csf3a (a.k.a. csf3, gcsf)
  • Promoter T7
  • Tag / Fusion Protein
    • (His)6 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site NcoI (unknown if destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHis-zfCSF3a was a gift from Petr Bartunek (Addgene plasmid # 65612 ; http://n2t.net/addgene:65612 ; RRID:Addgene_65612)
  • For your References section:

    Clonal analysis of hematopoietic progenitor cells in the zebrafish. Stachura DL, Svoboda O, Lau RP, Balla KM, Zon LI, Bartunek P, Traver D. Blood. 2011 Aug 4;118(5):1274-82. doi: 10.1182/blood-2011-01-331199. Epub 2011 Mar 17. 10.1182/blood-2011-01-331199 PubMed 21415264