pHis-zfEpoa
(Plasmid #65611)

• Purpose: Express soluble His-tagged zebrafish erythropoietin in E. coli
• Depositing Lab: Petr Bartunek
• Publication: Stachura et al Blood. 2009 Jul 9;114(2):279-89. doi: 10.1182/blood-2009-02-203638. Epub 2009 May 11.

Backbone

• Vector backbone: pETH2a
• Backbone size w/o insert (bp): 4500
• Total vector size (bp): 4989
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: The depositing laboratory recommends strain BL21(DE3) for protein expression.
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: erythropoietin
• Alt name: Epo
• Species: D. rerio (zebrafish)
• Insert Size (bp): 486
• GenBank ID: NM_001115128.1
• Entrez Gene: epoa (a.k.a. e, epo)
• Promoter: T7
• Tag / Fusion Protein:
  • (His)6 (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry