attB-GFP
(Plasmid
#65521)
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PurposePlasmid for the generation of GFP-knockin fusions via Bxb1-mediated recombination into MIN-tagged cell lines
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 65521 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC57
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Modifications to backboneGFP is split into two artifical exons separated by an intron harboring the Kanamycin resistance
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Vector typeMammalian Expression, Mouse Targeting ; Bxb1
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)JM109
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameattB-GFP
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Insert Size (bp)756
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
attB-GFP was a gift from Sebastian Bultmann (Addgene plasmid # 65521 ; http://n2t.net/addgene:65521 ; RRID:Addgene_65521) -
For your References section:
A modular open platform for systematic functional studies under physiological conditions. Mulholland CB, Smets M, Schmidtmann E, Leidescher S, Markaki Y, Hofweber M, Qin W, Manzo M, Kremmer E, Thanisch K, Bauer C, Rombaut P, Herzog F, Leonhardt H, Bultmann S. Nucleic Acids Res. 2015 May 24. pii: gkv550. 10.1093/nar/gkv550 PubMed 26007658
