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PurposeExpresses HBV polymerase in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 65520 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3
- Total vector size (bp) 8295
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHBV polymerase protein
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Alt nameHBx
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SpeciesHepatitis B virus
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Insert Size (bp)3466
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Tag
/ Fusion Protein
- Flag (N terminal on insert)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Pol was a gift from Wang-Shick Ryu (Addgene plasmid # 65520 ; http://n2t.net/addgene:65520 ; RRID:Addgene_65520) -
For your References section:
Hepatitis B virus polymerase suppresses translation of pregenomic RNA via a mechanism involving its interaction with 5' stem-loop structure. Ryu DK, Kim S, Ryu WS. Virology. 2008 Mar 30;373(1):112-23. Epub 2007 Dec 21. 10.1016/j.virol.2007.11.010 PubMed 18155120
Map uploaded by the depositor.
