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Addgene

pTre-Tight-luciferase-Hottip (522)
(Plasmid #65490)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65490 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTre-tight-SV40-luciferase
  • Backbone manufacturer
    Guigo lab, Addgene plasmid 65489
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Hottip
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    718
  • Entrez Gene
    HOTTIP (a.k.a. HOXA-AS6, HOXA13-AS1, NCRNA00213)
  • Promoter tight TRE promoter

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer C328F AGGCGTATCACGAGGCCCTTTCGT
  • 3′ sequencing primer C328R TATTACCGCCTTTGAGTGAGCTGA
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTre-Tight-luciferase-Hottip (522) was a gift from Roderic Guigo & Rory Johnson (Addgene plasmid # 65490 ; http://n2t.net/addgene:65490 ; RRID:Addgene_65490)
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