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Addgene

pEGFP_mNTAQ1
(Plasmid #65486)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65486 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Ntaq1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Wdyhv1 (a.k.a. 2410187C16Rik, AU014961, AW550036, Ntaq1)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (unknown if destroyed)
  • 3′ cloning site SacII (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP_mNTAQ1 was a gift from Alexander Varshavsky (Addgene plasmid # 65486 ; http://n2t.net/addgene:65486 ; RRID:Addgene_65486)

  • For your References section:

    Glutamine-specific N-terminal amidase, a component of the N-end rule pathway. Wang H, Piatkov KI, Brower CS, Varshavsky A. Mol Cell. 2009 Jun 26;34(6):686-95. doi: 10.1016/j.molcel.2009.04.032. 10.1016/j.molcel.2009.04.032 PubMed 19560421
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