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Addgene

pLENTI-PGK-HYGRO-GATA6mut-HA
(Plasmid #65476)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65476 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti PGK Hygro DEST
  • Backbone manufacturer
    Eric Campeau, Addgene plasmid 19066
  • Backbone size w/o insert (bp) 10301
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GATA6mut
  • Alt name
    GATA binding protein 6
  • Species
    H. sapiens (human)
  • Mutation
    lacking the eight most highly conserved bases of the zinc-finger motif
  • Entrez Gene
    GATA6
  • Promoter PGK
  • Tag / Fusion Protein
    • HA (C terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLENTI-PGK-HYGRO-GATA6mut-HA was a gift from Eduard Batlle (Addgene plasmid # 65476 ; http://n2t.net/addgene:65476 ; RRID:Addgene_65476)

  • For your References section:

    The transcription factor GATA6 enables self-renewal of colon adenoma stem cells by repressing BMP gene expression. Whissell G, Montagni E, Martinelli P, Hernando-Momblona X, Sevillano M, Jung P, Cortina C, Calon A, Abuli A, Castells A, Castellvi-Bel S, Nacht AS, Sancho E, Stephan-Otto Attolini C, Vicent GP, Real FX, Batlle E. Nat Cell Biol. 2014 Jul;16(7):695-707. doi: 10.1038/ncb2992. Epub 2014 Jun 22. 10.1038/ncb2992 PubMed 24952462
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