pKS011
(Plasmid #65464)

• Purpose: pSC101 based plasmid where pl-tetO expression drives synthesis of construct expressing (N to C terminal) MBP (Maltose Binding Protein), Ntag, and mCherry
• Depositing Lab: Keith Tyo
• Publication: Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. doi: 10.1371/journal.pone.0149746. eCollection 2016.

Backbone

• Vector backbone: pSC101
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: MBP-Ntag-mCherry
• Insert Size (bp): 2270
• Promoter: pl-TetO
• Tags / Fusion Proteins:
  • MBP
  • FLAG

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GGCGTATCACGAGGCCCTTTC
• 3′ sequencing primer: GGATAACAGAAAGGCCGGGAAATAC

Resource Information

• Supplemental Documents:
  • annotate GenBank file of pKS011
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry