-
Purposeexpresses HBx in mammalian cells
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 65463 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepCMV
- Total vector size (bp) 6763
-
Vector typeMammalian Expression
-
Selectable markersNeomycin (select with G418)
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberUnknown
Gene/Insert
-
Gene/Insert nameHBV X protein
-
Alt nameHBx
-
SpeciesHepatitis B virus
-
Insert Size (bp)462
-
Tag
/ Fusion Protein
- Flag (C terminal on insert)
Resource Information
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
HBx was a gift from Wang-Shick Ryu (Addgene plasmid # 65463 ; http://n2t.net/addgene:65463 ; RRID:Addgene_65463) -
For your References section:
Hepatitis B virus X protein is essential for the activation of Wnt/beta-catenin signaling in hepatoma cells. Cha MY, Kim CM, Park YM, Ryu WS. Hepatology. 2004 Jun;39(6):1683-93. 10.1002/hep.20245 PubMed 15185310