mVenus-PA-MKI
(Plasmid
#65457)
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Purposephotoactivatable myosin light-chain kinase inihibition
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 65457 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepVenus-C1
- Backbone size w/o insert (bp) 5200
- Total vector size (bp) 5230
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMKI
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Alt nameMLCKi
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SpeciesSynthetic
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Insert Size (bp)30
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F or GFP-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mVenus-PA-MKI was a gift from Klaus Hahn (Addgene plasmid # 65457 ; http://n2t.net/addgene:65457 ; RRID:Addgene_65457) -
For your References section:
Manipulation of endogenous kinase activity in living cells using photoswitchable inhibitory peptides. Yi JJ, Wang H, Vilela M, Danuser G, Hahn KM. ACS Synth Biol. 2014 Nov 21;3(11):788-95. doi: 10.1021/sb5001356. Epub 2014 Jun 13. 10.1021/sb5001356 PubMed 24905630