pITGFP3
(Plasmid
#65452)
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Purpose(Empty Backbone) pITGFP3 was designed to test the promoter activity using the destabilized yeast enhanced green fluorescent protein (yEGFP-CLN2PEST) as reporter.
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 65452 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepUC19
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Vector typeYeast Expression
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Tag
/ Fusion Protein
- yEGFP-CLN2PEST reporter
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pITGFP3 was a gift from Claudia Vickers (Addgene plasmid # 65452 ; http://n2t.net/addgene:65452 ; RRID:Addgene_65452) -
For your References section:
Controlling heterologous gene expression in yeast cell factories on different carbon substrates and across the diauxic shift: a comparison of yeast promoter activities. Peng B, Williams TC, Henry M, Nielsen LK, Vickers CE. Microb Cell Fact. 2015 Jun 26;14:91. doi: 10.1186/s12934-015-0278-5. 10.1186/s12934-015-0278-5 PubMed 26112740