pOPINB-OTUB1*
(Plasmid #65441)

• Purpose: Expresses activated OTUB1 in E. coli
• Depositing Lab: David Komander
• Publication: Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi: 10.1016/j.molcel.2015.01.042. Epub 2015 Mar 5.

Backbone

• Vector backbone: pOPINB
• Backbone manufacturer: OPPF-UK
• Backbone size w/o insert (bp): 5531
• Total vector size (bp): 6836
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: UbcH5b
• Species: H. sapiens (human)
• Mutation: Active site cysteine mutated to alanine
• Entrez Gene: UBE2D2 (a.k.a. E2(17)KB2, PUBC1, UBC4, UBC4/5, UBCH4, UBCH5B)
• Promoter: T7
• Tag / Fusion Protein:
  • His-3C (N terminal on backbone)

Cloning Information for Gene/Insert 1

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Gene/Insert 2

• Gene/Insert name: otubain-1
• Entrez Gene: OTUB1 (a.k.a. HSPC263, OTB1, OTU1)

Cloning Information for Gene/Insert 2

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry