pLPCX-Cx43-FS-IRES-GFP
(Plasmid #65438)

• Purpose: Mammalian expression of frameshift mutated Cx43 and EGFP separately driven by the ECMV IRES. For transfection or retroviral production
• Depositing Lab: Trond Aasen
• Publication: Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. doi: 10.1186/1478-811X-12-31.

Backbone

• Vector backbone: pLPCX
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 6264
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cx43
• Alt name: Connexin 43
• Alt name: GJA1
• Species: H. sapiens (human)
• Insert Size (bp): 2575
• Mutation: frameshift (FS) mutation 35delA
• GenBank ID: 2697
• Entrez Gene: GJA1 (a.k.a. AVSD3, CMDR, CX43, EKVP, EKVP3, GJAL, HLHS1, HSS, ODDD, PPKCA)
• Promoter: CMV
• Tag / Fusion Protein:
  • IRES-GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: LNCX-3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Initially cloned into pBluescript: Cx43 insert digested out with EcoRV from Addgene plasmid #27383 and IRES-GFP digested from pIRES2-EGFP Addgene plasmid 3178 from clontech using BamHI-NotI. Entire Cx43-IRES-GFP cloned from pBluescript into pLPCX using XhoI and NotI.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

FrameShift Mutation at EcoNI site of Cx43 (35delA) leading to out of frame synthesis after 11 amino acid and premature stop at amino acid 100.