pLPCX-Cx43-IRES-GFP
(Plasmid #65433)

• Purpose: Mammalian expression of Cx43 and EGFP seperately driven by the ECMV IRES. For transfection or retroviral production
• Depositing Lab: Trond Aasen
• Publication: Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. doi: 10.1186/1478-811X-12-31.

Backbone

• Vector backbone: pLPCX
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 6264
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cx43
• Alt name: Connexin 43
• Alt name: GJA1
• Species: H. sapiens (human)
• Insert Size (bp): 2576
• GenBank ID: 2697
• Entrez Gene: GJA1 (a.k.a. AVSD3, CMDR, CX43, EKVP, EKVP3, GJAL, HLHS1, HSS, ODDD, PPKCA)
• Promoter: CMV
• Tag / Fusion Protein:
  • IRES-GFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: LNCX-3

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Initially cloned into pBluescript: Cx43 insert digested out with EcoRV from Addgene plasmid #27383 and IRES-GFP digested from pIRES2-EGFP Addgene plasmid 3178 from clontech using BamHI-NotI. Entire Cx43-IRES-GFP cloned from pBluescript into pLPCX using XhoI and NotI.
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry