pEGFPC1-Intu
(Plasmid
#65430)
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PurposeFor mammalian expression of EGFP-mouse Inturned
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 65430 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFPC1
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Modifications to backboneNone
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameInturned
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SpeciesM. musculus (mouse)
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Entrez GeneIntu (a.k.a. 9230116I04Rik, 9430087H23Rik, Pdzd6, Pdzk6, mKIAA1284)
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Tag
/ Fusion Protein
- EGFP (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer ATG GTC CTG CTG GAG TTC GT
- 3′ sequencing primer GGGAGGTGTGGGAGGTTTT (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEGFPC1-Intu was a gift from Aimin Liu (Addgene plasmid # 65430 ; http://n2t.net/addgene:65430 ; RRID:Addgene_65430) -
For your References section:
PCP effector gene Inturned is an important regulator of cilia formation and embryonic development in mammals. Zeng H, Hoover AN, Liu A. Dev Biol. 2010 Mar 15;339(2):418-28. doi: 10.1016/j.ydbio.2010.01.003. Epub 2010 Jan 11. 10.1016/j.ydbio.2010.01.003 PubMed 20067783
