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Addgene

pTGFP-T7-Hha10comp
(Plasmid #65428)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65428 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTurboGFPN
  • Backbone manufacturer
    EvroGen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Prof. Timothy R. O'Connor (City of Hope) provided the original vector, i.e., pTGFP-Hha10, which were modified from pTurboGFPN plasmid (EvroGen, San Diego) . (Pubmed ID: 17507378).

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The pTGFP-Hha10 vector was used to generate the competitor vector, i.e., pTGFP-T7-Hha10comp.

Note: Addgene's quality control sequencing finds a T18N amino acid residue substitution in TurboGFP. This residue change is not thought to affect plasmid function for transcriptional mutagenesis assay.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTGFP-T7-Hha10comp was a gift from Yinsheng Wang (Addgene plasmid # 65428 ; http://n2t.net/addgene:65428 ; RRID:Addgene_65428)

  • For your References section:

    Quantitative measurement of transcriptional inhibition and mutagenesis induced by site-specifically incorporated DNA lesions in vitro and in vivo. You C, Wang Y. Nat Protoc. 2015 Sep;10(9):1389-406. doi: 10.1038/nprot.2015.094. Epub 2015 Aug 20. 10.1038/nprot.2015.094 PubMed 26292071
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