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Addgene

pHLSec2-FN-YPet
(Plasmid #65421)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65421 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHLSec2
  • Backbone size w/o insert (bp) 4600
  • Total vector size (bp) 12141
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human plasma fibronectin with YPet inserted between FNIII domains 6 and 7
  • Alt name
    fibronectin 1
  • Species
    H. sapiens (human)
  • Entrez Gene
    FN1 (a.k.a. CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, SMDCF)
  • Tag / Fusion Protein
    • YPet

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHLSec2-FN-YPet was a gift from Harold Erickson (Addgene plasmid # 65421 ; http://n2t.net/addgene:65421 ; RRID:Addgene_65421)

  • For your References section:

    Fibronectin aggregation and assembly: the unfolding of the second fibronectin type III domain. Ohashi T, Erickson HP. J Biol Chem. 2011 Nov 11;286(45):39188-99. doi: 10.1074/jbc.M111.262337. Epub 2011 Sep 23. 10.1074/jbc.M111.262337 PubMed 21949131
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