pAAV-CaMKIIa-HA-KORD-IRES-mCitrine
(Plasmid #65418)

• Purpose: AAV packaging vector for CamKIIa driven KOR DREADD expression
• Depositing Lab: Bryan Roth
• Publication: Vardy et al Neuron. 2015 Apr 29. pii: S0896-6273(15)00289-5. doi: 10.1016/j.neuron.2015.03.065.

Backbone

• Vector backbone: pAAV2
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CaMKIIa-HA-KORD-IRES-mCitrine
• Alt name: CamKIIa KOR DREADD
• Species: H. sapiens (human)
• Entrez Gene: OPRK1 (a.k.a. K-OR-1, KOP, KOR, KOR-1, KOR1, OPRK)
• Promoter: CaMKIIa
• Tags / Fusion Proteins:
  • HA (N terminal on insert)
  • mCitrine (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: Unknown
• 3′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • pAAV-CaMKIIa-HA-KORD-IRES-mCitrine
• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

These plasmids were generated as part of the Illuminating the Druggable Genome (IDG) program sponsored by the NIH Common Fund. The goal of this program is to identify, gather, and distribute information and resources for proteins that currently are not well-studied yet belong to commonly drug-targeted protein families: protein kinases, non-olfactory G-protein coupled receptors (GPCRs), and ion channels. The IDG program is designed to develop fundamental research tools for understudied proteins, elucidate their function, and disseminate the IDG-related resources and data to the greater scientific community.