pBS-HxB.S
(Plasmid
#65415)
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Purposecontains complete cDNA for TNC small splice variant flanked by NotI-KpnI
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 65415 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBS (probably KS+)
- Backbone size w/o insert (bp) 2961
- Total vector size (bp) 8342
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nametenascin C small splice variant
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SpeciesH. sapiens (human)
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Insert Size (bp)5381
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Entrez GeneTNC (a.k.a. 150-225, DFNA56, GMEM, GP, HXB, JI, TN, TN-C)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site KpnI (not destroyed)
- 5′ sequencing primer cat ggtcatagct gtttcc
- 3′ sequencing primer tc gaggtcgacg gtatc (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: Plasmid contains the Q680R natural variant within tenascin C small splice variant.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS-HxB.S was a gift from Harold Erickson (Addgene plasmid # 65415 ; http://n2t.net/addgene:65415 ; RRID:Addgene_65415) -
For your References section:
Cell- and heparin-binding domains of the hexabrachion arm identified by tenascin expression proteins. Aukhil I, Joshi P, Yan Y, Erickson HP. J Biol Chem. 1993 Feb 5;268(4):2542-53. PubMed 7679097
