Skip to main content
Addgene

RCAN1
(Plasmid #65413)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65413 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    p3Xflag-CMV
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5450
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RCAN1
  • Alt name
    MCIP1
  • Alt name
    regulator of calcineurin 1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    756
  • Entrez Gene
    Rcan1 (a.k.a. Adapt78, CALP1L, CSP1, DSC1, Dscr1, MCIP1, RCN1)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag tagged (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RCAN1 was a gift from Jeffery Molkentin (Addgene plasmid # 65413 ; http://n2t.net/addgene:65413 ; RRID:Addgene_65413)

  • For your References section:

    Interaction between TAK1-TAB1-TAB2 and RCAN1-calcineurin defines a signalling nodal control point. Liu Q, Busby JC, Molkentin JD. Nat Cell Biol. 2009 Feb;11(2):154-61. doi: 10.1038/ncb1823. Epub 2009 Jan 11. 10.1038/ncb1823 PubMed 19136967
Related items:
Commonly requested with: