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Addgene

GFP-TRIM66
(Plasmid #65400)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65400 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA6.2/N-EmGFP-DEST
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 5847
  • Vector type
    Mammalian Expression
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TRIM66
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3471
  • Mutation
    none
  • Entrez Gene
    TRIM66 (a.k.a. C11orf29, TIF1D, TIF1DELTA)
  • Promoter CMV
  • Tags / Fusion Proteins
    • GFP (N terminal on backbone)
    • V5 (C terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer EGFP_C_primer
  • 3′ sequencing primer TK polyA reverse primer
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Gene Source: HT1080 cDNA

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-TRIM66 was a gift from Kyle Miller (Addgene plasmid # 65400 ; http://n2t.net/addgene:65400 ; RRID:Addgene_65400)

  • For your References section:

    Screen identifies bromodomain protein ZMYND8 in chromatin recognition of transcription-associated DNA damage that promotes homologous recombination. Gong F, Chiu LY, Cox B, Aymard F, Clouaire T, Leung JW, Cammarata M, Perez M, Agarwal P, Brodbelt JS, Legube G, Miller KM. Genes Dev. 2015 Jan 15;29(2):197-211. doi: 10.1101/gad.252189.114. 10.1101/gad.252189.114 PubMed 25593309
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