GFP-TRIM33
(Plasmid #65398)

• Purpose: GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
• Depositing Lab: Kyle Miller
• Publication: Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. doi: 10.1101/gad.252189.114.

Backbone

• Vector backbone: pcDNA6.2/N-EmGFP-DEST
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 5847
• Vector type: Mammalian Expression
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TRIM33
• Alt name: TIF1-gamma
• Species: H. sapiens (human)
• Insert Size (bp): 3381
• Mutation: none
• Entrez Gene: TRIM33 (a.k.a. ECTO, PTC7, RFG7, TF1G, TIF1G, TIF1GAMMA, TIFGAMMA)
• Promoter: CMV
• Tags / Fusion Proteins:
  • EmGFP (N terminal on backbone)
  • V5 (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: EGFP_C_primer
• 3′ sequencing primer: TK polyA reverse primer

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Gene Source: Addgene 15734