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PurposeGFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 65390 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA6.2/C-EmGFP-DEST
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Backbone manufacturerinvitrogen
- Backbone size w/o insert (bp) 5761
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Vector typeMammalian Expression
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameSMARCA2
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Alt nameBRM
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Alt nameBAF190B
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SpeciesH. sapiens (human)
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Insert Size (bp)4716
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Mutationnone
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Entrez GeneSMARCA2 (a.k.a. BAF190, BIS, BRM, NCBRS, SNF2, SNF2L2, SNF2LA, SWI2, Sth1p, hBRM, hSNF2a)
- Promoter CMV
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Tag
/ Fusion Protein
- EmGFP (C terminal on backbone)
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer T7_promoter_primer
- 3′ sequencing primer EGFP_N_primer (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Gene Source: DNASU HsCD00082837
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SMARCA2-GFP was a gift from Kyle Miller (Addgene plasmid # 65390 ; http://n2t.net/addgene:65390 ; RRID:Addgene_65390) -
For your References section:
Screen identifies bromodomain protein ZMYND8 in chromatin recognition of transcription-associated DNA damage that promotes homologous recombination. Gong F, Chiu LY, Cox B, Aymard F, Clouaire T, Leung JW, Cammarata M, Perez M, Agarwal P, Brodbelt JS, Legube G, Miller KM. Genes Dev. 2015 Jan 15;29(2):197-211. doi: 10.1101/gad.252189.114. 10.1101/gad.252189.114 PubMed 25593309