POT5-CYC1p-ycrtYB-ADH1t
(Plasmid
#65360)
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PurposeTU for combinatorial construction of ?-carotene pathway
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 65360 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePOT5
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Vector typeUnspecified
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCYC1p-ycrtYB-ADH1t
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Insert Size (bp)2518
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13 Reverse (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
construction of TUs(CYC1p-ycrtYB-ADH1t) in POT5 receiving vector
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
POT5-CYC1p-ycrtYB-ADH1t was a gift from Junbiao Dai (Addgene plasmid # 65360 ; http://n2t.net/addgene:65360 ; RRID:Addgene_65360) -
For your References section:
YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J. Nucleic Acids Res. 2015 May 8. pii: gkv464. 10.1093/nar/gkv464 PubMed 25956650