HCKan-O-ycrtE
(Plasmid
#65344)
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Purposeconstruction of ycrtE gene in HCKan-O receiving vector
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 65344 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneHCKan-O
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameycrtE
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SpeciesSynthetic
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Insert Size (bp)1127
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer pBR322ori-3
- 3′ sequencing primer Kan-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HCKan-O-ycrtE was a gift from Junbiao Dai (Addgene plasmid # 65344 ; http://n2t.net/addgene:65344 ; RRID:Addgene_65344) -
For your References section:
YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J. Nucleic Acids Res. 2015 May 8. pii: gkv464. 10.1093/nar/gkv464 PubMed 25956650