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pRS414-URR
(Plasmid #65329)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65329 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS414
  • Backbone manufacturer
    Agilent Technologies
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    URR1-RFP-Leu2-URR2
  • Species
    Synthetic
  • Insert Size (bp)
    4421

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

pRS414 with URR1-RFP-Leu2-URR2 frament inserted at MCS, used for receiving in vitro ligated pathway by gap-repair method(URR1 and URR2 are homologous arms)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRS414-URR was a gift from Junbiao Dai (Addgene plasmid # 65329 ; http://n2t.net/addgene:65329 ; RRID:Addgene_65329)

  • For your References section:

    YeastFab: the design and construction of standard biological parts for metabolic engineering in Saccharomyces cerevisiae. Guo Y, Dong J, Zhou T, Auxillos J, Li T, Zhang W, Wang L, Shen Y, Luo Y, Zheng Y, Lin J, Chen GQ, Wu Q, Cai Y, Dai J. Nucleic Acids Res. 2015 May 8. pii: gkv464. 10.1093/nar/gkv464 PubMed 25956650
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