Str-STIM1-NN_neomycin
(Plasmid #65311)

• Purpose: Luminal ER hook only (RUSH system)
• Depositing Lab: Franck Perez
• Publication: Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.

Backbone

• Vector backbone: pIRESneo3
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: STIM1 mutated for binding motif to microtubule
• Species: H. sapiens (human)
• Mutation: 2 amino acids of STIM1 (IP from SxIP motif) were mutated to NN to abolish microtubule binding
• Entrez Gene: STIM1 (a.k.a. D11S4896E, GOK, IMD10, STRMK, TAM, TAM1)
• Promoter: CMV
• Tag / Fusion Protein:
  • streptavidin (N terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: CMV Forward
• 3′ sequencing primer: synth-int-R

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

luminal tagging