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PurposeLuminal ER hook only (RUSH system)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 65306 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepIRESneo3
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Backbone manufacturerClontech
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameStreptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
- Promoter CMV
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Tags
/ Fusion Proteins
- Signal Sequence (N terminal on insert)
- KDEL motif (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer synth-int-R (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
luminal tagging
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Str-KDEL_neomycin was a gift from Franck Perez (Addgene plasmid # 65306 ; http://n2t.net/addgene:65306 ; RRID:Addgene_65306) -
For your References section:
Synchronization of secretory protein traffic in populations of cells. Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche L, Mercanti V, Jollivet F, Raposo G, Perez F. Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928. 10.1038/nmeth.1928 PubMed 22406856
