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pCDH_Str-KDEL_ManII-SBP-mCherry
(Plasmid #65259)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65259 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCDH-CMV-MCS-EF1-puro
  • Backbone manufacturer
    system biosciences
  • Vector type
    Lentiviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Streptavidin-KDEL and truncated Mannosidase II
  • Alt name
    MAN2A1
  • Species
    M. musculus (mouse)
  • Mutation
    contains only amino acids 1 to 116 of ManII
  • Promoter CMV
  • Tags / Fusion Proteins
    • Streptavidin Binding Protein (SBP) (C terminal on insert)
    • mCherry (C terminal on insert)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

luminal tagging

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDH_Str-KDEL_ManII-SBP-mCherry was a gift from Franck Perez (Addgene plasmid # 65259 ; http://n2t.net/addgene:65259 ; RRID:Addgene_65259)

  • For your References section:

    Synchronization of secretory protein traffic in populations of cells. Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche L, Mercanti V, Jollivet F, Raposo G, Perez F. Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928. 10.1038/nmeth.1928 PubMed 22406856
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